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Before You Redesign a Coding Sequence: Seven Checks for Recombinant Expression
A coding sequence can be changed substantially at the DNA level while still encoding the same protein. This is possible because multiple codons can specify the same amino acid. When researchers prepare a gene for recombinant expression, they may consider redesigning synonymous codons to better fit a selected host system. However, the expression host is only one part of the design decision. A...
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